Make sure your camera sensor is as clean as possible before you start.Failure to allow enough overlap causes banding to appear in the stacked output. This is important for the stacking software to work properly. Allow plenty of overlap between steps the idea is to move smoothly though the depth of the subject just as though you were focusing normally.If you have good parfocality between your visual eyepieces and your camera, and your trinocular head permits simultaneous viewing and photography, then it is possible to judge steps by eye, otherwise use the tick marks on your fine focus and be careful not to loose concentration and forget which direction you are turning the focus wheel!.Check visually that the size of focus step you have chosen is appropriate to the subject and magnification.Stacking works best with a series of approximately equal focus steps between top and bottom settings.
bottom → top or top → bottom, not randomly.
COST OF ZERENE FOCUS STACKER HOW TO
Practise your stacking technique on subjects that don’t move! Live subjects like water fleas and mosquito larvae make attractive subjects for stacking, but you need to learn how to confine or anaesthetise them first.Stacking inevitably has its limitations, and there are a few general rules that will help you to make better stacked images.
COST OF ZERENE FOCUS STACKER FULL
What if we took a new picture each time we made an adjustment to the fine focus, and then combined each of these ‘slices’ together to reconstruct the full depth of the subject? In fact we can do exactly this and use software to select the sharply-focused parts of each slice and combine them into a single composite image showing greatly extended apparent depth of field. That way, we get a much better impression of the 3D structure we are seeing. To appreciate them, we use the fine-focus control to bring various parts into sharp focus in sequence. Many of the subjects that we enjoy examining through our microscope have significant depth. Stacking is the process of combining a series of digital images of the same subject area, taken at different focal planes, into a single image with great depth of field, using a computer program. Home | Resources | Stacking & stitching | Stacking Stacking Enhancing depth of field by combining image stacks Introduction
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